TABLE 1.
Response of T84 cells to anthrax EdTxa
| Toxin | Cell surface applied and treatment | Lag (min) | dIsc/dt (μA/cm2/min) | Peak Isc (μA/cm2) |
|---|---|---|---|---|
| WT PA | ||||
| n = 28 | basolateral | 17 ± 7 | 0.7 ± 0.5 | 28 ± 17 |
| n = 8 | apical | >80 | ND | 1.2 ± 0.2 |
| WT PA (trypsin treated) | ||||
| n = 7 | basolateral | 20 ± 4 | 0.3 ± 0.1 | 17 ± 6b |
| n = 9 | apical | >80 | ND | 1.4 ± 0.4 |
| PASSSR, n = 8 | basolateral | >80 | ND | 1.5 ± 0.5 |
| PA--D | basolateral | >80 | ND | 1.9 ± 0.6 |
| WT PA, n = 8 | basolateral; Baf A1 | >80 | ND | 1.7 ± 0.7 |
a
Values are reported as means ± standard deviations. ND, not detectable.
b
Trypsin-treated EdTx induced a slightly lower Isc than did whole EdTx (30% reduction), as has been observed previously (33).