Fig. 4.
Effects of aNK cell infusion on senescence markers p16 (A) and β-galactosidase (B) for human PBMCs in vivo. Donors D and E were infused with 2 × 109 of aNK cells twice, as indicated by the upper row (Days 0 through 192, first infusion) and lower row (Days 0 through 267, second infusion) of the x-axis label. Total study period 459 days. Error bar = Standard deviation. Data was obtained in duplicates for each donor at each time point. Data within each time point were pooled from the two donors and analyzed with Kruskal-Wallis test followed by post-hoc Dunn's test. Expression of senescent markers p16 and β-galactosidase decreases following infusion of aNK cells and trends toward pre-infusion levels by 6 months post-infusion; the pattern is repeated with a second infusion. Significant differences were denoted by *: 0.01 < p ≤ 0.05; **: 0.001 < p ≤ 0.01; ***: p ≤ 0.001. If not indicated, results are not statistically significant.