Table 2. Relative Rates of Substrates with Intact Enzyme, Split Constructs, and Autocomplementation^a.

Enzyme	Nitrocefin	CENTA	Fluorocillin	C-5Fu
G3-TEM1	6.44 ± 0.0559	4.71 ± 0.0312	9.0 × 10–3 ± 8.8 × 10–5	0.73 ± 0.11
βN-9.29 + G3-βC	1.81 ± 0.0156	0.099 ± 0.00038	7.4 × 10–4 ± 8.5 × 10–5	0.033 ± 0.0018
βN + G3-βC	0.034 ± 4.7 × 10–5	4.2 × 10–3 ± 4.8 × 10–4	4.9 × 10–5 ± 4.6 × 10–9
βN-9.29 + G3-βC (no HER2)	0.033 ± 4.7 × 10–5	9.1 × 10–3 ± 1.9 × 10–4	9.9 × 10–5 ± 6.2 × 10–9

^aSlopes adjusted assuming maximum signal indicate complete substrate turnover, and turnover is given in nmol/min. Note: C-5Fu turnover was calculated using 750 nM enzyme, and the other substrates used 100 nM enzyme. Mean ± SD, N = 3.