Figure 3.

Discovery and identification of EGCG as an inhibitor targeting NADK. (A) Chemical structures of several candidate compounds that were screened from an in-house compound library. (B) Determination of the IC₅₀ of EGCG using the enzymatic activity assay. (C) Carr–Purcell–Meiboom–Gill data from the incubation of 200 μΜ EGCG with 10 μM NADK. (D) Determination of the affinity of EGCG and NADK by the SPR assay. (E) EGCG is a noncompetitive inhibitor of NADK with K_i = 3.28 ± 0.32 μΜ. Data were fitted using GraphPad Prism software in noncompetitive mode. The IC₅₀, K_d, and K_i values are shown as mean ± SD.