Table 1. in Vitro Biological Activity of Amplified Hits and Acarbose.
| IC50 (μM) |
|||||
|---|---|---|---|---|---|
| entry | compound | α-glucosidase | α-amylase | CC50 (μM) | amplification folda |
| 1 | C1E3 | >200 | >200 | 1.59 | |
| 2 | C2E3 | >200 | >200 | 2.10 | |
| 3 | C3E3 | 167.98 ± 5.59 | >200 | 4.86 | |
| 4 | C3E5 | 32.53 ± 2.38 | >200 | >200 | 1.95 |
| 5 | C4E5 | 6.49 ± 0.53 | 128.10 ± 4.62 | >200 | 2.68 |
| 6 | C4E6 | 22.60 ± 1.12 | 151.46 ± 5.69 | >200 | 2.13 |
| 7 | C4E8 | 2.52 ± 0.21 | >200 | >200 | 2.32 |
| 8 | acarbose | 8.06 ± 0.33 | 5.32 ± 0.21 | ||
a
Calculated as (%P/%B), where %P and %B were the relative peak areas of the compounds in the HPLC spectra of the protein-templated reaction and the blank reaction, respectively.