Table 3. Potency of Compound 25 against hNa_V1.1–1.8, Ca_V1.2, and hERG¹⁷.

isoform	IC50 (μM)a	no. of cells
hNaV1.1	>100	3
hNaV1.2	>100	3
hNaV1.3	65.3, 62.7–68.1	3
hNaV1.4	80.7, 71.1–93.3	14
hNaV1.5	>100	9
hNaV1.6	17.9, 14.8–22.1	13
hNaV1.7	0.072, 0.064–0.082	25
hNaV1.8	>100	3
CaV1.2	>100	2
hERG	>100	2

^aIC₅₀ determined by manual patch clamp reported as mean; 95% confidence interval.