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. 2022 Sep 8;9(32):2204067. doi: 10.1002/advs.202204067

Figure 3.

Figure 3

Active transcytosis of PRNs contributes to deeper tumor penetration. A) Confocal images showing the penetration behavior of NRNs and PRNs into MCSs at pH 6.7 and pH 7.4. B) MFI of MCSs calculated by ImageJ. C) Scheme showing PRN penetration in MCSs. PRNs can penetrate into deep region of MCSs through enhanced active transcytosis and passive diffusion. D) Scheme showing the process of real‐time observation of PRN microdistribution in tumors. E) In vivo real‐time observation of the microdistribution of PRNs and NRNs in 4T1 tumors after intravenous (i.v.) administration. F) Time‐dependent MFI changes of PRNs in the extravascular tumor tissues. G) Confocal images of typical tumor tissue sections after i.v. injection of PRNs for 12 h. The tumor vessels were labelled by CD31‐FITC (green) and nuclei were labelled by DAPI (blue). H) Profiles showing the fluorescence intensity along the solid yellow lines on the panel (G). I) MFI of Cy5 in tumor tissues after treatment of PRNs. Data are shown as mean ± SD by one‐way analysis of variance (ANOVA) or unpaired two‐tailed Student's t‐tests were used (n = 3). **p < 0.005, ***p < 0.001, ****p < 0.0001.