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. 2022 Sep 8;9(32):2204067. doi: 10.1002/advs.202204067

Figure 4.

Figure 4

PRNs enhanced ICD performance and subsequent immune stimulation in deep tumor tissue. A) Scheme showing that the tumor cells undergoing ICD after PRNs plus NIR‐II laser irradiation treatment release immunogenic DAMPs and promote DC maturation. B) Confocal images showing the exposure of CALR and the translocation of HMGB1 in 4T1 cells at 4 and 20 h after NIR‐II laser irradiation. MFI of C) CALR exposure and D) HMGB1 translocation from nuclei to cytoplasm in 4T1 tumor cells. E,F) Flow cytometry analysis (FACS) of activation of purified murine bone marrow derived dendritic cells (BMDCs) after culturing with dying 4T1 cells for 12 h. Expression of E) CD80 and F) CD86 on BMDCs. Data are shown as mean ± SD (n = 3). G) Immunofluorescence staining of CALR exposure and HMGB1 release in 4T1 tumors at 4 and 20 h post NIR‐II laser irradiation. H) Corresponding quantifications of the MFI of CALR. I) The PRNs plus NIR‐II laser irradiation can induce massive release of DAMPs in the deeper depth of 4T1 tumors and induce stronger DC activation. Data are shown as mean ± SD by one‐way analysis of variance (ANOVA) or unpaired two‐tailed Student's t‐tests were used (n = 3). **p < 0.005, ***p < 0.001, ****p < 0.0001.