Figure 5.

Effective inhibition on established subcutaneous 4T1 tumor model by P RN^MT plus NIR‐II laser irradiation. A) Schematic showing the process of antitumor treatments. Tumor cells were inoculated 8 days before injecting therapeutic agents. The tumor volume was measured every 2 days (DOSE of 20 mg CuS per kilogram mouse weight, laser power was 1 W cm⁻²). Data are shown as mean ± SD (n = 5), *p < 0.05, **p < 0.005, ***p < 0.001. B) Tumor volume evolution during the treatments. C) Weights of tumor excised at the end of therapy. D) Mice weight changes during the treatment. E–H) After 18 days, the mice were sacrificed and spleens were harvested, and the percentages of E) CD4⁺ T cells, F) CD8⁺ T cells, G) CD62L⁺ CD4⁺ T cells, H) CD62L⁺ CD8⁺ T cells were measured by flow cytometry (n = 6). Immunofluorescence staining of Foxp3⁺ (I) and CD8⁺ (K) T cells. Quantifications of Foxp3⁺ (J) and CD8⁺ (L) area in panels (I) and (K). Data are shown as mean ± SD by one‐way analysis of variance (ANOVA) or two‐way ANOVA with Tukey's multiple comparisons test (n = 5). **p < 0.005, ***p < 0.001, ****p < 0.0001.