Figure 8.

Cytosolic phospholipase A2 (cPLA2), a nuclear mechanosensor, responds to changes in cell geometry. A) The nuclear shape index and nuclear perimeter in micropatterned SMCs were calculated. B) Representative three‐dimensional reconstruction images of the nucleus of micropatterned cells and the quantification of the nuclear surface area. Cells were infected with pSIN‐H2B‐tagGFP viruses. C) Representative fluorescent images of cPLA2‐EYFP fusion proteins in micropatterned cells. Cells were transfected with the cPLA2‐EYFP plasmids before seeding. D) Representative immunofluorescent staining of cPLA2 and nuclear envelope protein lamin B1 in micropatterned cells. Z1–Z3 indicate three different scanning layers. The distribution of cPLA2 and lamin B1 and the degree of overlap between them are indicated by fluorescence intensity profiling (right panels). E) Production of arachidonic acid (ArAc) in the micropatterned cells was assayed using ELISA. Data were obtained from eight biological repeats. In (A, B) (right panel), each dot represents a single cell. In (E), data were from eight biological repeats. Significance was assessed using a Student's t‐test. The error bars show ± SD. The exact P values between the indicated groups are presented.