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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Methods Enzymol. 2022 May 12;674:363–389. doi: 10.1016/bs.mie.2022.04.008
Equipment Source Catalog number
1. Nunc 10cm petri dish (untreated) Thermo Fisher Scientific 150239
2. 12 well-treated sterile plates Thermo Fisher Scientific 07-200-81
3. 10mL serological pipettes
25mL serological pipettes
Thermo Fisher Scientific 1367811 E
1367811
4. Nalgene Oak Ridge high-speed polycarbonate centrifuge tubes Thermo Fisher Scientific 3118-0050-PK
5. CO2 incubator Thermo Electron Corporation 3110
6. Brightline hemocytometer Thermo Fisher Scientific 0267110
7. SterilGARD Biosafety level II laminar flow hood The Baker company Inc. SG 600
8. Centrifuges Eppendorf
Eppendorf
5417 R
5810 R
9. Sorvall-RC-5B refrigerated superspeed centrifuge Du Pont instruments RC5B 9001008
10. Fisher vortex Genie 2 Thermo Fisher Scientific 12-812
11. Automatic environmental speed-Vac system Savant Instruments Inc. AES 1010-120
12. High-performance liquid chromatography (HPLC) system with Empower software Alliance equipped with a 2998 photodiode array detector (Waters) Waters e2695 Separations Module
13. Zorbax RX-SIL (5μm 4.6 × 150mm column) Agilent 883975-901
14. BD Precisionglide® syringe needles gauge 18, L 1 in. 305,195 and 25, L 1in. 305,125 BD Biosciences 148265 G and 1482113 D
15. 22g × 25mm curved gavage needle Thermo Fisher Scientific NC 1197789
16. Surgical forceps and scissors
17. Fisherbrand Cell Lifters Thermo Fisher Scientific 08-100-240
18. BD Vacutainer SST venous blood collection tube (8.5mL) BD Biosciences 367988
19. Standard IVC cage for blood collection (11½” × 7¼”) Allentown 227422
20. Millipore express plus 0.22μM PES steritop filter Millipore Sigma S-2-GPT-05-RE
21. Nanodrop 2000 spectrophotometer Thermo Fisher Scientific R-052
22. GeneAmp PCR System 9700 Applied Biosystems 805-S-6061302
23. StepOnePlus real-time PCR System Thermo Fisher Scientific (Applied Biosystems) 2720013071
24. Beckman L7-80 ultracentrifuge Beckman Inc. 80-M-9208
25. SW-41 Ti rotor Beckman Inc. 6826
26. Polypropylene ultracentrifuge tubes (14 × 89 mm) Beckman Inc. 331372
27. Polyvinylidene fluoride (PVDF) membrane (0.2μm; 26.5cm × 37.5cm) Thermo Fisher Scientific 88520
28. Glass plates for electrophoresis Bio-Rad 1655308
1653312
29. Bio-Rad Power pac 200 Bio-Rad 284-BR-04521