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. Author manuscript; available in PMC: 2022 Nov 14.
Published in final edited form as: Methods Enzymol. 2022 May 12;674:363–389. doi: 10.1016/bs.mie.2022.04.008
Reagent Source Catalog number
1. Gibco DMEM, high glucose Thermo Fisher Scientific 11965118
2. Fetal bovine serum Thermo Fisher Scientific 10437028
3. Penicillin–streptomycin Thermo Fisher Scientific MT-30-002-CI
4. l-Glutamine Thermo Fisher Scientific MT-25-005-CI
5. RBC lysis buffer 1 × Thermo Fisher Scientific 00-4333-57
6. Trypsin–EDTA (0.25%, phenol red) Thermo Fisher Scientific 25200114
7. Thioglycolate medium brewer modified (dehydrated culture media) BD BBL BD 211716
8. Trypan blue Gibco 15250061
9. Ethanol Thermo Fisher Scientific 4355223
10. Acetone Thermo Fisher Scientific A 184
11. Hexane Thermo Fisher Scientific H 3024
12. Ethyl acetate Thermo Fisher Scientific E 1954
13. Retinyl palmitate Cayman Chemicals 23796
14. Retinyl acetate Cayman Chemicals 20242
15. Retinol Sigma R 7632
16. Phosphate-buffered saline (PBS, 10 ×, pH 7.4) Thermo Fisher Scientific BP 3991
17. Dimethyl sulfoxide (DMSO) Sigma D 2650
18. Pierce Coomassie Plus (Bradford) Assay Kit Thermo Fisher Scientific 23236
19. AIN 93G-Purified diet Research Diets D 17041301
20. Alanine transaminase and aspartate aminotransferase activity kits Abcam ab 105134 and ab 105135
21. Pierce chromogenic endotoxin quant kit Thermo Fisher Scientific A 39552
22. TRIzol LS Reagent Thermo Fisher Scientific 10296010
23. Direct-zol RNA MicroPrep (200 Preps) with Zymo-Spin IC Columns Zymo Research R 2063
24. Verso cDNA synthesis reverse transcriptase kit Thermo Fisher Scientific AB 1453 B
25. Mouse Hprt probe Thermo Fisher Scientific Mm-03024075_m1
26. Mouse Rarß probe Thermo Fisher Scientific Mm-01319677_m1
27. Mouse Cyp26a1 probe Thermo Fisher Scientific Mm-00514486_m1
28. Mouse Ccl2 probe Thermo Fisher Scientific Mm-00441242_m1
29. Taqman fast advance mix Thermo Fisher Scientific 44-449-63
30. Sudan black Sigma S-0395
31. Ethylene glycol Thermo Fisher Scientific E-178-1
32. Potassium bromide Thermo Fisher Scientific P-205-500
33. Sucrose Thermo Fisher Scientific S5-500
34. 30% Acrylamide Bio-Rad 1610158
35. 2× Laemmli buffer Bio-Rad 1610737
36. Ammonium persulfate Sigma A-3678
37. Tetramethyl ethylenediamine (TEMED) Sigma 110-18-9
38. anti-Apolipoprotein B (ApoB, Polyclonal) Millipore Sigma AB-742
39. Polyclonal rabbit anti-human retinol-binding protein (anti-RBP4) Dako A-0040
40. Retinoic acid Sigma R2500