TABLE 1.
Superoxide production in response to PMA and concentrations of IFN-γ and TNF-α in the supernatants of PBMC and EHM cultured with medium only (controls), IL-12 of 100 ng/ml, anti-IFN-γ, or both IL-12 at 100 ng/ml and anti-IFN-γ for 2 to 3 days
| Cells and culture supplement(s) | Mean concn ± SEM fora:
|
||
|---|---|---|---|
| IFN-γ (pg/ml) | TNF-α (pg/ml) | O2− (nmol/106 MNC/15 min) | |
| PBMC | |||
| None (control) | 83 ± 42 | 71 ± 26 | 1.3 ± 0.2 |
| IL-12 | 1,000 ± 172* | 167 ± 23† | 2.2 ± 0.3§ |
| Anti-IFN-γ | 16 ± 13 | 89 ± 32 | 1.4 ± 0.2 |
| IL-12 + anti-IFN-γ | 11 ± 5 | 134 ± 62‡ | 2.1 ± 0.3¶ |
| EHM | |||
| None (control) | 5 ± 1 | 6 ± 1 | 3.6 ± 0.7 |
| IL-12 | 3 ± 0 | 4 ± 0 | 6.2 ± 1.1** |
The values for IFN-γ and TNF-α concentrations are means ± SEMs of duplicate readings of four supernatants. The values for O2− production by PBMC and EHM are means ± SEMs derived from 20 or 21 and 8 or 9 experiments, respectively. *, P = 0.03 in comparison to the value for control PBMC; †, P = 0.045 in comparison to the value for control PBMC; §, P = 0.04 in comparison to the value for control PBMC; ‡ P = 0.55 in comparison to the value for anti-IFN-γ-treated PBMC; ¶, P = 0.03 in comparison to the value for anti-IFN-γ-treated PBMC; **, P = 0.013 in comparison to the value for control EHM.