Figure 1.

Study design The saliva-coated hydroxyapatite disc, consisting of similar components as in tooth enamel, was used as the substrate for biofilm formation. C. albicans and S. mutans were grown alone or together in 0.1% sucrose without disturbance until 24-h for initial biofilm establishment. The culture medium was changed once daily. To induce a cariogenic challenge, the culture medium was changed to 1% sucrose at 24-h and 48-h. The inoculation quantity of C. albicans (10³ CFU/ml) and S. mutans (10⁵ CFU/ml) was chosen to mimic high caries risk conditions in the clinical setting.