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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Am J Hematol. 2022 Sep 26;97(12):1548–1559. doi: 10.1002/ajh.26716

Figure 1. Intracellular iron regulates Bmp6 transcription in primary mouse liver endothelial cells.

Figure 1.

(A-C) Primary liver endothelial cells were treated with solvent (Solv) or iron-chelator deferoxamine (DFO, 100 μM) for 24 hours, or serum-starved for 8 hours before treatment with apo-or holo-transferrin (TF, 30 μM) for 24 hours and analyzed for: (A) Western blot and quantitation of iron storage protein ferritin light (L) chain relative to ß-actin as a loading control; (B-C) qRT-PCR of Tfrc and Bmp6 relative to Rpl19 expression. (D-E) Primary mouse liver endothelial cells were treated with 40 nM control (Ctrl) siRNA or siRNA targeting Tfrc for 48 hours, serum-starved for 8 hours, treated with apo- or holo-TF in serum-free media for 24 hours, and analyzed for: (D) Western blot and quantitation of ferritin heavy chain (FTH1) relative to ß-actin; (E) qRT-PCR of Bmp6 relative to Rpl19 expression. Representative blots from N=4 independent experiments shown. Bar graphs represent mean ± SEM with individual points indicating the number of replicates. Statistical differences were determined by (A-C) two-tailed Student’s t-test or (D-E) one-way ANOVA with Holm-Sidak method of multiple comparisons.