Figure 5.

Targeting latent TGF-β of AsPC-1-TGF tumor cells induces AdCAR T cell activation and does not interfere with targeting a TAA. (A) Architecture of AdCAR1 and AdCAR2. Primary human T cells expressing AdCAR1 or AdCAR2 were either co-cultured with (B) AsPC-1 WT or (C) AsPC-1-TGF for 141 h in presence of indicated mixtures of aLAP and aCD66c. The surface area in wells covered by target cells normalized to the start value was quantified with the IncuCyte® S3 system. (D) T cells were co-cultured with supernatant of AsPC-1-TGF for 24 h in presence of indicated mixtures of aLAP and aCD66c. MFI of CD137⁺ cells was analyzed. Data shown were obtained from (n=3) independent donors measured in duplicates if not indicated differently and plotted with mean and ± 1 (SD). Statistical analysis was performed with Two-way ANOVA. Correction for multiple comparisons was done with Holm-Sidak correction.