Figure 6.

Autologous PBMCs synergized with AdCAR T cells and improved functionality in the AsPC-1-TGF tumor model. (A) Schematic representation of the study design and measurement of serum IFN-γ levels on day 4 to assess soluble antigen sensing in mice reconstituted with autologous PBMCs and inoculated with AsPC-1-TGF. (B) Quantification of AdCAR2-mediated cytotoxicity by total BLI. Median of each cohort (n≥5) is shown. (C) Ex vivo analysis of viable CD45⁺ cells in tumor tissue and of (D) viable CD8⁺ T cells as well as (E) exhaustion marker expression of T cells in spleens and tumor tissues (n=4). Ex vivo analysis was performed on day 25 for all cohorts. Statistical analysis was performed with ordinary one-way ANOVA (A) or Two-way ANOVA (B,D). Correction for multiple comparisons was done with Holm-Sidak correction. Median is plotted as dashed line. Each dot represents one animal. Experiments were performed with cells of one donor.