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. 2022 Dec 1;13(6):1901–1918. doi: 10.14336/AD.2022.0316

Figure 1.

Figure 1.

Age-dependent changes in vasodilation function, and eNOS, p53, p21 p16 cGAS, STING and p-IRF3/IRF3 expression levels in mice. Acetylcholine (ACh)-induced relaxation (A) and sodium nitroprusside (SNP)-induced relaxation (B) of mouse aortas were measured in the 2 Months, 6 Months, 12 Months and 24 Months age groups. (C) The protein levels of eNOS, p53, p21, p16, cGAS, STING, p-IRF3, IRF3 and β-actin were measured in the aortas of mice in each age group by western blot analysis. β-actin was used as the housekeeper protein for normalization. Quantification of protein levels is shown in (D). Data were analyzed by (A and B) two-way ANOVA or (D) one way ANOVA plus Bonferroni post hoc test. All data shown are mean±SD. AU indicates arbitrary units. Relative expression is the fold changes relative to the 2 Months group. n=6, *P<0.05 compared with the 2 Months group.