TABLE 3.
Absolute and relative weight of major organs from SD rats orally treated with lac dye for 90 days.
| Dose of lac dye (mg/kg) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 50 | 100 | 200 | 500 | |||||||||
| Male (n = 10/group) | |||||||||||||
| Liver | (g) | 16.52 ± 2.34 | 13.79 ± 0.77* | 14.72 ± 1.88 | 15.02 ± 2.21 | 14.33 ± 1.42* | |||||||
| (g%) | 2.89 ± 0.23 | 2.62 ± 0.15* | 2.64 ± 0.19* | 2.76 ± 0.26 | 2.67 ± 0.16 | ||||||||
| Spleen | (g) | 0.99 ± 0.22 | 0.88 ± 0.16 | 0.94 ± 0.14 | 0.90 ± 0.14 | 0.83 ± 0.06 | |||||||
| (g%) | 0.17 ± 0.03 | 0.17 ± 0.03 | 0.17 ± 0.02 | 0.17 ± 0.02 | 0.16 ± 0.01 | ||||||||
| Kidney (R) | (g) | 1.71 ± 0.17 | 1.62 ± 0.16 | 1.68 ± 0.21 | 1.67 ± 0.16 | 1.62 ± 0.17 | |||||||
| (g%) | 0.30 ± 0.03 | 0.31 ± 0.03 | 0.30 ± 0.03 | 0.31 ± 0.02 | 0.30 ± 0.04 | ||||||||
| Kidney (L) | (g) | 1.72 ± 0.21 | 1.61 ± 0.17 | 1.68 ± 0.21 | 1.66 ± 0.18 | 1.59 ± 0.17 | |||||||
| (g%) | 0.30 ± 0.03 | 0.31 ± 0.03 | 0.30 ± 0.03 | 0.31 ± 0.02 | 0.30 ± 0.03 | ||||||||
| Adrenal gl. (R) | (g) | 0.030 ± 0.005 | 0.029 ± 0.004 | 0.031 ± 0.006 | 0.027 ± 0.005 | 0.029 ± 0.003 | |||||||
| (g%) | 0.005 ± 0.001 | 0.006 ± 0.001 | 0.006 ± 0.001 | 0.005 ± 0.001 | 0.005 ± 0.001 | ||||||||
| Adrenal gl. (L) | (g) | 0.033 ± 0.005 | 0.031 ± 0.005 | 0.032 ± 0.005 | 0.030 ± 0.004 | 0.030 ± 0.004 | |||||||
| (g%) | 0.006 ± 0.001 | 0.006 ± 0.001 | 0.006 ± 0.001 | 0.006 ± 0.001 | 0.006 ± 0.001 | ||||||||
| Testis (R) | (g) | 1.75 ± 0.12 | 1.81 ± 0.21 | 1.77 ± 0.13 | 1.76 ± 0.07 | 1.85 ± 0.08 | |||||||
| (g%) | 0.31 ± 0.03 | 0.34 ± 0.04 | 0.32 ± 0.03 | 0.33 ± 0.03 | 0.35 ± 0.03* | ||||||||
| Testis (L) | (g) | 1.81 ± 0.14 | 1.79 ± 0.20 | 1.80 ± 0.13 | 1.75 ± 0.08 | 1.90 ± 0.10 | |||||||
| (g%) | 0.32 ± 0.03 | 0.34 ± 0.04 | 0.33 ± 0.03 | 0.32 ± 0.03 | 0.36 ± 0.03 | ||||||||
| Epididymis(R) | (g) | 0.75 ± 0.05 | 0.75 ± 0.07 | 0.73 ± 0.08 | 0.73 ± 0.04 | 0.77 ± 0.09 | |||||||
| (g%) | 0.13 ± 0.01 | 0.14 ± 0.01 | 0.13 ± 0.02 | 0.14 ± 0.01 | 0.14 ± 0.02 | ||||||||
| Epididymis(L) | (g) | 0.78 ± 0.04 | 0.74 ± 0.08 | 0.72 ± 0.06 | 0.70 ± 0.03* | 0.81 ± 0.07 | |||||||
| (g%) | 0.14 ± 0.01 | 0.14 ± 0.01 | 0.13 ± 0.01 | 0.13 ± 0.01 | 0.15 ± 0.02 | ||||||||
| Prostate and Coagulating gl | (g) | 3.52 ± 0.30 | 3.56 ± 0.33 | 3.46 ± 0.46 | 3.52 ± 0.41 | 3.60 ± 0.62 | |||||||
| (g%) | 0.62 ± 0.10 | 0.68 ± 0.07 | 0.62 ± 0.10 | 0.65 ± 0.09 | 0.67 ± 0.11 | ||||||||
| Heart | (g) | 1.67 ± 0.13 | 1.53 ± 0.09 | 1.65 ± 0.18 | 1.59 ± 0.17 | 1.59 ± 0.09 | |||||||
| (g%) | 0.29 ± 0.01 | 0.29 ± 0.02 | 0.30 ± 0.03 | 0.29 ± 0.02 | 0.30 ± 0.02 | ||||||||
| Lung | (g) | 1.68 ± 0.14 | 1.56 ± 0.10 | 1.70 ± 0.19 | 1.61 ± 0.10 | 1.61 ± 0.09 | |||||||
| (g%) | 0.30 ± 0.01 | 0.30 ± 0.02 | 0.31 ± 0.02 | 0.30 ± 0.02 | 0.30 ± 0.02 | ||||||||
| Thymus | (g) | 0.30 ± 0.05 | 0.24 ± 0.03 | 0.28 ± 0.07 | 0.26 ± 0.08 | 0.26 ± 0.05 | |||||||
| (g%) | 0.05 ± 0.01 | 0.05 ± 0.01 | 0.05 ± 0.01 | 0.05 ± 0.01 | 0.05 ± 0.01 | ||||||||
| Thyroid (R) | (g) | 0.015 ± 0.003 | 0.015 ± 0.003 | 0.016 ± 0.002 | 0.015 ± 0.003 | 0.015 ± 0.002 | |||||||
| (g%) | 0.003 ± 0.000 | 0.003 ± 0.000 | 0.003 ± 0.000 | 0.003 ± 0.001 | 0.003 ± 0.000 | ||||||||
| Thyroid (L) | (g) | 0.016 ± 0.003 | 0.015 ± 0.003 | 0.016 ± 0.003 | 0.015 ± 0.002 | 0.014 ± 0.002 | |||||||
| (g%) | 0.003 ± 0.000 | 0.003 ± 0.001 | 0.003 ± 0.001 | 0.003 ± 0.000 | 0.003 ± 0.000 | ||||||||
| Brain | (g) | 2.31 ± 0.13 | 2.21 ± 0.08 | 2.26 ± 0.12 | 2.22 ± 0.08 | 2.28 ± 0.09 | |||||||
| (g%) | 0.41 ± 0.03 | 0.42 ± 0.03 | 0.41 ± 0.03 | 0.41 ± 0.02 | 0.43 ± 0.03 | ||||||||
| Pituitary gl | (g) | 0.0143 ± 0.0016 | 0.0131 ± 0.0022 | 0.0136 ± 0.0018 | 0.0136 ± 0.0017 | 0.0133 ± 0.0011 | |||||||
| (g%) | 0.0025 ± 0.0004 | 0.0025 ± 0.0004 | 0.0025 ± 0.0004 | 0.0025 ± 0.0003 | 0.0025 ± 0.0002 | ||||||||
| Parotid gl. (R) | (g) | 0.16 ± 0.03 | 0.14 ± 0.02 | 0.15 ± 0.02 | 0.14 ± 0.02 | 0.14 ± 0.02 | |||||||
| (g%) | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | ||||||||
| Parotid gl. (L) | (g) | 0.15 ± 0.03 | 0.14 ± 0.02 | 0.14 ± 0.02 | 0.14 ± 0.01 | 0.14 ± 0.01 | |||||||
| (g%) | 0.03 ± 0.01 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | 0.03 ± 0.00 | ||||||||
| Submaxillary gl. (R) | (g) | 0.41 ± 0.04 | 0.39 ± 0.03 | 0.40 ± 0.04 | 0.42 ± 0.04 | 0.41 ± 0.04 | |||||||
| (g%) | 0.07 ± 0.00 | 0.07 ± 0.00 | 0.07 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | ||||||||
| Submaxillary gl. (L) | (g) | 0.42 ± 0.03 | 0.39 ± 0.04 | 0.4 ± 0.04 | 0.41 ± 0.04 | 0.41 ± 0.03 | |||||||
| (g%) | 0.07 ± 0.00 | 0.07 ± 0.01 | 0.07 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | ||||||||
| 0 | 50 | 100 | 200 | 500 | |||||||||
| Female (n = 10/group) | |||||||||||||
| Liver | (g) | 9.46 ± 1.14 | 8.87 ± 1.61 | 9.30 ± 0.86 | 9.23 ± 1.29 | 9.46 ± 1.12 | |||||||
| (g%) | 2.83 ± 0.35 | 2.72 ± 0.28 | 2.87 ± 0.27 | 2.90 ± 0.31 | 2.89 ± 0.20 | ||||||||
| Spleen | (g) | 0.57 ± 0.06 | 0.57 ± 0.08 | 0.58 ± 0.07 | 0.61 ± 0.10 | 0.56 ± 0.09 | |||||||
| (g%) | 0.17 ± 0.02 | 0.18 ± 0.03 | 0.18 ± 0.02 | 0.19 ± 0.03 | 0.17 ± 0.02 | ||||||||
| Kidney (R) | (g) | 1.02 ± 0.06 | 0.98 ± 0.11 | 0.98 ± 0.11 | 1.04 ± 0.09 | 1.03 ± 0.08 | |||||||
| (g%) | 0.31 ± 0.03 | 0.30 ± 0.02 | 0.30 ± 0.03 | 0.33 ± 0.04 | 0.31 ± 0.02 | ||||||||
| Kidney (L) | (g) | 0.98 ± 0.07 | 0.96 ± 0.11 | 0.94 ± 0.08 | 1.00 ± 0.08 | 1.01 ± 0.11 | |||||||
| (g%) | 0.29 ± 0.03 | 0.30 ± 0.02 | 0.29 ± 0.03 | 0.32 ± 0.03 | 0.31 ± 0.02 | ||||||||
| Adrenal gl. (R) | (g) | 0.034 ± 0.005 | 0.033 ± 0.004 | 0.033 ± 0.003 | 0.034 ± 0.004 | 0.035 ± 0.005 | |||||||
| (g%) | 0.010 ± 0.002 | 0.010 ± 0.002 | 0.010 ± 0.001 | 0.011 ± 0.002 | 0.011 ± 0.002 | ||||||||
| Adrenal gl. (L) | (g) | 0.037 ± 0.005 | 0.034 ± 0.004 | 0.034 ± 0.004 | 0.037 ± 0.004 | 0.037 ± 0.005 | |||||||
| (g%) | 0.011 ± 0.002 | 0.011 ± 0.002 | 0.011 ± 0.002 | 0.012 ± 0.002 | 0.011 ± 0.002 | ||||||||
| Ovary (R) | (g) | 0.040 ± 0.005 | 0.043 ± 0.010 | 0.039 ± 0.007 | 0.043 ± 0.008 | 0.034 ± 0.009 | |||||||
| (g%) | 0.012 ± 0.001 | 0.013 ± 0.003 | 0.012 ± 0.003 | 0.013 ± 0.002 | 0.010 ± 0.003 | ||||||||
| Ovary (L) | (g) | 0.041 ± 0.009 | 0.040 ± 0.011 | 0.042 ± 0.006 | 0.041 ± 0.011 | 0.036 ± 0.009 | |||||||
| (g%) | 0.012 ± 0.002 | 0.012 ± 0.003 | 0.013 ± 0.002 | 0.013 ± 0.003 | 0.011 ± 0.003 | ||||||||
| Uterus | (g) | 0.77 ± 0.23 | 0.74 ± 0.33 | 0.97 ± 0.66 | 0.75 ± 0.16 | 0.87 ± 0.36 | |||||||
| (g%) | 0.23 ± 0.06 | 0.24 ± 0.12 | 0.30 ± 0.22 | 0.24 ± 0.07 | 0.27 ± 0.12 | ||||||||
| Heart | (g) | 1.06 ± 0.11 | 0.99 ± 0.09 | 1.03 ± 0.10 | 1.04 ± 0.08 | 1.00 ± 0.08 | |||||||
| (g%) | 0.32 ± 0.02 | 0.31 ± 0.03 | 0.32 ± 0.03 | 0.33 ± 0.03 | 0.31 ± 0.03 | ||||||||
| Lung | (g) | 1.28 ± 0.09 | 1.28 ± 0.09 | 1.30 ± 0.10 | 1.32 ± 0.14 | 1.29 ± 0.16 | |||||||
| (g%) | 0.38 ± 0.04 | 0.40 ± 0.05 | 0.40 ± 0.04 | 0.42 ± 0.08 | 0.40 ± 0.06 | ||||||||
| Thymus | (g) | 0.27 ± 0.09 | 0.23 ± 0.07 | 0.27 ± 0.09 | 0.27 ± 0.08 | 0.24 ± 0.06 | |||||||
| (g%) | 0.08 ± 0.02 | 0.07 ± 0.02 | 0.08 ± 0.03 | 0.09 ± 0.02 | 0.07 ± 0.02 | ||||||||
| Thyroid (R) | (g) | 0.012 ± 0.002 | 0.013 ± 0.004 | 0.012 ± 0.003 | 0.013 ± 0.003 | 0.013 ± 0.002 | |||||||
| (g%) | 0.004 ± 0.001 | 0.004 ± 0.001 | 0.004 ± 0.001 | 0.004 ± 0.001 | 0.004 ± 0.001 | ||||||||
| Thyroid (L) | (g) | 0.011 ± 0.002 | 0.011 ± 0.002 | 0.013 ± 0.002 | 0.012 ± 0.003 | 0.011 ± 0.001 | |||||||
| (g%) | 0.003 ± 0.001 | 0.003 ± 0.001 | 0.004 ± 0.001 | 0.004 ± 0.001 | 0.003 ± 0.001 | ||||||||
| Brain | (g) | 1.95 ± 0.10 | 2.05 ± 0.09 | 2.03 ± 0.07 | 2.07 ± 0.09* | 1.99 ± 0.10 | |||||||
| (g%) | 0.59 ± 0.06 | 0.64 ± 0.07 | 0.63 ± 0.06 | 0.65 ± 0.08 | 0.61 ± 0.06 | ||||||||
| Pituitary gl | (g) | 0.0181 ± 0.0024 | 0.0201 ± 0.0044 | 0.0174 ± 0.0038 | 0.0204 ± 0.0024 | 0.0220 ± 0.0050 | |||||||
| (g%) | 0.0054 ± 0.0008 | 0.0062 ± 0.0010 | 0.0054 ± 0.0012 | 0.0065 ± 0.0011 | 0.0068 ± 0.0017* | ||||||||
| Parotid gl. (R) | (g) | 0.12 ± 0.02 | 0.12 ± 0.02 | 0.12 ± 0.02 | 0.12 ± 0.03 | 0.12 ± 0.02 | |||||||
| (g%) | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | ||||||||
| Parotid gl. (L) | (g) | 0.12 ± 0.02 | 0.11 ± 0.02 | 0.12 ± 0.02 | 0.13 ± 0.03 | 0.12 ± 0.02 | |||||||
| (g%) | 0.04 ± 0.00 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | ||||||||
| Submaxillary gl. (R) | (g) | 0.25 ± 0.03 | 0.23 ± 0.02 | 0.25 ± 0.03 | 0.25 ± 0.02 | 0.25 ± 0.03 | |||||||
| (g%) | 0.07 ± 0.01 | 0.07 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | ||||||||
| Submaxillary gl. (L) | (g) | 0.25 ± 0.02 | 0.24 ± 0.03 | 0.25 ± 0.03 | 0.25 ± 0.02 | 0.25 ± 0.03 | |||||||
| (g%) | 0.07 ± 0.01 | 0.07 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | 0.08 ± 0.01 | ||||||||
*p < 0.05 by one-way ANOVA, followed by post hoc Dunnett’s t-test.