TABLE 2.
Antibodies and transformation constructs used to localize amebic organelles
| Structure | Antibodies to: | Constructs |
|---|---|---|
| Secretory | Chitinasea | Chitinase |
| Vesicles | Ariel | |
| ER | BiP-myc, chitinase (+) KDEL | |
| Cytosol | ADH1 | Chitinase (−) signal |
| Golgi | Mammalian ARF, mammalian ɛ-COP |
a
Encystation-specific chitinase in E. invadens. All constructs were in the pJST4 vector and transfected into E. histolytica trophozoites. In addition, pinosomes were localized with FITC-dextran, phagosomes with GFP-labeled bacteria, and lysosomes with the fluorescent substrate (Arg–Arg–4-methoxy-2-naphthylamide).