TABLE 2.
Effect of 48 h influenza B virus infection of human nasopharyngeal explants on subsequent bacterial association by N. meningitidis 6 and 24 h after inoculation
| Treatment of explant, period of superinfection with N. meningitidis | Viable N. meningitidis (CFU [103] ± SEM) recovered per mg of homogenized explant (n = 7)
|
SEM (n = 6)
|
TEM (n = 6)
|
||||
|---|---|---|---|---|---|---|---|
| After washing alone | After washing plus bile treatment | Invasive fraction | No. of explantsa | Mean no. of bacteria in sample area (0.06 mm2) | No. of explantsb | Ratio of bacteria to epithelial cells in section | |
| Influenza B virus for 48 h | |||||||
| 6 h | 3.43 ± 0.99 | 0.28 ± 0.13 | 0.17 ± 0.06 | 4 | 86 ± 35 | 1 | 0.012 |
| 24 h | 48.74 ± 10.93 | 7.67 ± 4.44 | 0.11 ± 0.06 | 6 | 150 ± 47 | 3 | 0.042 ± 0.015 |
| Uninfected control at 48 h | |||||||
| 6 h | 3.43 ± 1.05 | 0.13 ± 0.05 | 0.05 ± 0.02 | 6 | 381 ± 170 | 2 | 0.021 ± 0.008 |
| 24 h | 157.46 ± 81.32 | 3.91 ± 1.94 | 0.03 ± 0.01 | 6 | 555 ± 172 | 3 | 0.516 ± 0.457 |
a
No. of explants with N. meningitidis on epithelial surface.
b
No. of explants displaying N. meningitidis on or within epithelium.