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| Components | Concentrations | Incubation time |
|---|---|---|
| Capture antibody | 3 μg mL−1 | Overnight |
| COVID-19 spike Ag | 2 μg mL−1 | 120 minutes |
| Primary antibody | 2 μg mL−1 | 120 minutes |
| Biotinylated secondary antibody | 0.032 μg mL−1 | 60 minutes |
| ZIF-8 encapsulated TA2-Gluc | 1 μM | 10 minutes |
