Figure 7. The larger the experimental uncertainty, the larger the affinity range required for a given $R_{\mathit{max}}^2$.

Corresponding to Equation 4, the maximum achievable R² for a given dataset is limited by the range of affinities and the associated experimental uncertainty. The illustration assumes that $\sigma$(measurement error) and $\sigma$(affinity) are in the same units, with an experimental error of 0.64 kcal mol⁻¹ indicated.