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. 2022 Sep 2;2(6):620–628. doi: 10.1021/acsmeasuresciau.2c00050

Table 1. NCAP-1 Autolysis Product Identifications.

peak number deconvoluted mass (g/mol) theoretical mass (g/mol)a NCAP-1 residues cleavage sequence
1 4169.97 4169.97 2–40 KQR | RPQ
2 3885.83 3885.81 2–38 RSK | QRR
3 3670.68 3670.68 2–36 GAR | SKQ
4 9071.23 9071.23 381–463 ETQ | ALP
5 7564.37 7564.37 394–463 TVT | LLP
6 8249.74 8249.74 388–463 RQK | KQQ
7 8121.65 8121.65 389–463 QKK | QQT
         
main NCAP-1 elution peak 19917.9 19917.9 2–185 SSR | SSS
  30437.8 30437.6 186–463
  20578.6 20578.6 2–191 RSR | NSS
  29776.8 29776.9 192–463
  46183.8 46183.2 41–463 KQR | RPQ
  50337.9 50337.5 2–463 intact N
a

Values for peaks 1–7 are monoisotopic masses; main NCAP-1 peak species are average masses.