Skip to main content
. 2021 Aug 6;81(20):5268–5283. doi: 10.1158/0008-5472.CAN-21-0494

Figure 7.

Figure 7. WTAPP1 RNA is a therapeutic target in mouse PDAC xenografts. A, Timeline schematic for treatment of mice carrying PDAC xenograft in the pancreas with siWTAPP1. Colored arrows indicate the times when different events occurred. B and C, Administration of siWTAPP1 via tail vein significantly suppressed tumor growth and metastasis. Shown are the radiant images of xenograft in mice (N = 5; B) and tumor growth rate expressed as radiance intensity (C). D, Survival times of mice treated with or without siWTAPP1. E, Timeline schematic for treatment of mice with PDX. Colored arrows indicate the times when different events occurred. F, Shown are the images of PDXs from three patients in 5 mice. G, Tumor growth curves (top) and tumor volumes in the end of experiment (bottom), with administration of siWTAPP1 via tail vein. H–J, Effects of siWTAPP1 treatment on WTAPP1 RNA level determined by qRT-PCR (H), WTAP protein level analyzed by Western blotting (I), and the ability of EIF3B bound to WTAP mRNA detected by EIF3B RIP-qPCR (J). R in I stands for the same positive reference sample for loading adjustment on each gel. Each protein band was semiquantified by gray density and the value for each band is relative to density of corresponding band of R. Data in G represent mean ± SD and other measurements are mean ± SEM. **, P < 0.01; ***, P < 0.001; ns, not significant of Student t test.

WTAPP1 RNA is a therapeutic target in mouse PDAC xenografts. A, Timeline schematic for treatment of mice carrying PDAC xenograft in the pancreas with siWTAPP1. Colored arrows indicate the times when different events occurred. B and C, Administration of siWTAPP1 via tail vein significantly suppressed tumor growth and metastasis. Shown are the radiant images of xenograft in mice (N = 5; B) and tumor growth rate expressed as radiance intensity (C). D, Survival times of mice treated with or without siWTAPP1. E, Timeline schematic for treatment of mice with PDX. Colored arrows indicate the times when different events occurred. F, Shown are the images of PDXs from three patients in 5 mice. G, Tumor growth curves (top) and tumor volumes in the end of experiment (bottom), with administration of siWTAPP1 via tail vein. H–J, Effects of siWTAPP1 treatment on WTAPP1 RNA level determined by qRT-PCR (H), WTAP protein level analyzed by Western blotting (I), and the ability of EIF3B bound to WTAP mRNA detected by EIF3B RIP-qPCR (J). R in I stands for the same positive reference sample for loading adjustment on each gel. Each protein band was semiquantified by gray density and the value for each band is relative to density of corresponding band of R. Data in G represent mean ± SD and other measurements are mean ± SEM. **, P < 0.01; ***, P < 0.001; ns, not significant of Student t test.