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. 2022 Nov 15;132(22):e154229. doi: 10.1172/JCI154229

Figure 1. Characterization of a GEMM of G34R pHGG.

Figure 1

(A) Procedure to induce genetically engineered H3.3-G34R pHGG in mice. Neonatal murine brain stem cells were transfected in vivo with SB transposase integration sequences to incorporate pHGG-inducing genetic lesions into the cells, including H3.3-G34R expression. pHGG development was monitored in vivo by luminescence driven by luciferase expression, and mice were perfused once signs of pHGG burden appeared; tumor tissue can be identified by its red (G34R) and green (ATRX-KO) fluorescence. Scale bars: 2 mm. DPI, days post implantation. (B) Illustration of the transposable fragments of the plasmids used to induce H3.3-G34R pHGG in mice via SB transposition. (C) Survival of animals transfected in vivo to develop de novo H3.3-G34R pHGG. (D) Selection of differentially enriched GOs between H3.3-G34R and H3.3-WT de novo–induced mouse pHGG, arranged by NES.