Figure 1.

Curcumin (Cur) rescued HG-induced osteogenic dysfunction of BMSCs. (a) Cell viability was determined by CCK-8 in the presence of different concentration of Cur for 24 h. (b)–(f) Osteogenic differentiation of BMSCs treated with Cur and different concentrations of glucose were determined with (b) ALP staining and ALP activity assays; (c) alizarin red staining and calcium deposition analysis; (d) expression of osteogenic-specific genes were assessed with qRT-PCR; (e)–(f) the expression of Runx2 by western blot. Data are presented as the mean ± SD from at least three independent experiments. ^Δp < 0.05, ^ΔΔp < 0.01, versus 0 μM Cur group. ^∗p < 0.05 and ^∗∗p < 0.01 versus NG group. ^#p < 0.05 and ^##p < 0.01 versus HG group.