FIG. 1.

RT-PCR of T-cell surface marker mRNA expression during primary C. albicans vaginal infection. Total RNA extracted from vaginal tissue or lymph nodes (three mice per group) was subjected to RT-PCR with primers specific for CD3, CD4, CD8, TCR-β constant region, and TCR-δ constant region of systemically derived T cells, using normal or HE Taq DNA polymerase. GAPDH served as the housekeeping gene. (A) RT-PCR of T-cell surface marker mRNA expression in lymph nodes and vaginal tissue of naive mice. (B) Semiquantitative RT-PCR of CD4, TCR-β, TCR-δ, and CD3 during a primary vaginal C. albicans infection. Results over time are expressed as the ratio of each product in pixel intensities to that for GAPDH. Est., estrogen-treated uninfected mice; Est./Inf., estrogen-treated infected mice; Inf., non-estrogen-treated infected mice; beta (b), β-chain; delta (d), δ-chain. The experiment was repeated twice, and the results shown are representative.