Fig. 3. Human ALL cell lines are susceptible to pharmacological inhibition of the PI3K/AKT/MTOR signaling pathway.

Titration curves for human leukemia cells cultured with increasing concentrations of (A) torin-1, (B) capivasertib and (C) everolimus. Viable cells were counted with trypan blue exclusion assay after four days. Data represent mean ± SEM of three independent experiments. D Graph shows means of half inhibitory growth concentration (IC50) of three independent experiments. E Titration curves of torin-1 for colony forming assay using murine E2A-PBX1⁺/PreBCR⁺ leukemia cells. Data represent mean ± SEM of three independent experiments. CFU, colony forming units. F Kaplan–meier curve represent disease-free survival of recipient mice transplanted with murine E2A-PBX1⁺/PreBCR⁺ leukemia cells after sublethal irradiation and treatment with vehicle or torin-1 (20 mg/kg b.w./d) starting at day 8 after transplantation. Each cohort contains 5 mice. Statistical analysis was performed by log-rank test.