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. 2022 Jun 27;29(11):1731–1741. doi: 10.1038/s41417-022-00492-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Genomic locus of circ_0006156. The expression of circ_0006156 was detected by qRT–PCR followed by Sanger sequencing. Arrows represent divergent primers binding to the back-splicing site of circ_0006156. B qRT–PCR analysis of the expression of circ_0006156 and FNDC3B mRNA after treatment with RNase R in PCa cells. C qRT–PCR analysis of the expression of circ_0006156 and FNDC3B mRNA after treatment with actinomycin D at the indicated time points in PCa cells. D qRT–PCR products with divergent primers showing circularization of circ_0006156 in PCa cells. cDNA, complementary DNA. gDNA, genomic DNA. E Cytoplasmic and nuclear mRNA fractionation experiments showed that circ_0006156 localized in both the nucleus and cytoplasm in DU145 cells. ACTB and U6 were applied as positive controls in the cytoplasm and nucleus, respectively. F RNA fluorescence in situ hybridization for circ_0006156 in DU145 cells. Nuclei were stained with DAPI.