Extended Data Fig. 8. CMTM4 has a substantial role in propagating experimental psoriasis, but not EAE.
a,b, Analysis of experimental psoriasis disease progression in Cmtm4+/+ (WT) or Cmtm4−/− littermate mice treated with Aldara cream containing 5% imiquimod (IMQ) on the shaven back and ears daily for five consecutive days and monitored daily. n = 14 mice per group in 3 independent experiments. Photograph of mice on day 4 (a) and mean ± s.e.m. for change of ear thickness, two-tailed Mann–Whitney test (b). c, Real-time quantitative PCR analysis of the relative expression of Il23, Il17a, Il17f, Il17c, and Cmtm4 transcripts in the back skin isolated from Cmtm4+/+ or Cmtm4−/− littermate mice treated or not with Aldara cream for four consecutive days. n = 4 for control groups, and 8 for IMQ-treated groups. Mean ± s.e.m., two-tailed Mann–Whitney test. d, Analysis of experimental psoriasis disease progression in wild-type mice that were transplanted with bone marrow isolated from Cmtm4+/+ or Cmtm4−/− mice. The experiment was performed as in a. Score for erythema, scaling, dorsal skin thickness, and combined cumulative score and changes in ear thickness are shown. n = 16 mice per group in 2 independent experiments analyzed. Mean ± s.e.m., two-tailed Mann–Whitney test. e, Analysis of the expression of IL-17RA, IL-17RC, CMTM4, and IL-17A mRNA in skin samples from patients with psoriasis (lesional or nonlesional samples) or healthy donors using the Genevestigator software. Each dot represents log2 of the average expression in the given sample. GEO accession numbers are GSE121212 for dataset HS-2913 and GSE54456 for dataset HS-1529. Two-tailed Mann–Whitney test. n.s., not significant. f, Clinical scores after MOG35–55-induced EAE. Mice were monitored daily in a blinded manner. n = 12 (Cmtm4+/+) or 14 (Cmtm4−/−). Mean ± s.e.m., two-way ANOVA.