Fig. 3. IL-17RC glycosylation is required for trafficking to the plasma membrane.
a, Immunoblotting analysis of lysates of ST2 cells transduced with expression vectors coding for SF-IL-17RC harboring indicated mutations of the putative glycosylation sites. b, Flow cytometry analysis of IL-17RC surface expression in cells expressing indicated SF-IL-17RC constructs. c, Immunoblotting analysis of samples isolated through Flag IP from lysates of ST2 cells expressing indicated SF-IL-17RC constructs. d–i, Immunoblotting (d,g), flow cytometry of surface IL-17RC (e,h), and fluorescence microscopy (f,i) in Cmtm4−/− ST2 cells expressing IL-17RC-EGFP only (d,e,f) or together with CMTM4-mCherry (g,h,i). Scale bar, 5 µm. Data are representative of three (a–c,f,i) or two (d,g) independent experiments. Flow cytometry data show mean + s.e.m. from three (b) or five (e,h) independent experiments. Two-tailed Mann–Whitney test (e,h) or two-tailed unpaired t-test (b).