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. 2022 Oct 21;23(11):1644–1652. doi: 10.1038/s41590-022-01325-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, b, Flow cytometry analysis of ST2 cells incubated with the indicated concentration of SF-IL-17A. Ligand binding was detected by Flag-APC antibody (a). Relative SF-IL-17A binding to ST2 cells was calculated from three independent experiments, mean ± s.e.m. (b). c, Immunoblotting analysis of lysates of wild-type ST2 cells that were stimulated with indicated concentrations of SF-IL-17A or commercial IL-17A (R&D Systems) for 15 min. d, Immunoblotting analysis of samples isolated through Flag immunoprecipitation from lysates of ST2 cells expressing SF-IL-17RB, SF-IL-17RD, or SF-IL-17RE. e, Comparison between murine and human IL-17RA and IL-17RC transmembrane domains. f, Flow cytometry analysis of surface expression of SF-IL-17RC^WT and SF-IL-17RC^TmRA ectopically expressed in ST2 cells. Mean + s.e.m. from six independent experiments. Two-tailed Mann–Whitney test. n.s. not significant. MFI, median fluorescence intensity. Data are representative of three (a–d) independent experiments.

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