. 2022 Nov 8;16:100499. doi: 10.1016/j.fochx.2022.100499

Table 3.

Phospholipid composition (μmol/g wet tissue) in total lipid extracted from control and protease hydrolysed hoki roe homogenate samples.

PL	Control	Inc	Alcalase			FP-II protease			HT protease			SEM	p value
			1 %	2 %	4 %	1 %	2 %	4 %	1 %	2 %	4 %		E	%EC	*E EC**
PA	0.58^A	0.61 ^A	0.44^BC	0.55^AB	0.34^CD	0.26^D	0.33^CD	ND	0.64^A	0.46^BC	0.44^BC	0.05	0.001	0.001	0.001
LDPG	0.71^BC	0.64^B	0.38^BC	0.30^BC	0.08^BC	0.21^BC	0.08^C	2.05^A	0.47^BC	0.39^BC	0.41^BC	0.15	0.004	0.001	0.001
CL	0.28	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	ND	NS	NS	NS
LPE	0.18^BC	0.21^AB	0.18^BC	0.23^AB	ND	ND	ND	ND	0.33^A	ND	0.13^C	0.03	0.001	0.002	0.001
LPS	5.34^A	5.42^A	2.87^CDE	2.87^DE	2.31^E	2.47^C	2.31^E	ND	4.03^BCD	3.42^CDE	2.95^DE	0.25	0.001	0.001	0.001
SM	1.24	0.47	ND	ND	ND	ND	ND	ND	ND	ND	ND	0.14	NS	NS	NS
PE	1.04	0.70	ND	ND	ND	ND	ND	ND	ND	ND	ND	0.13	NS	NS	NS
LPC	2.50^C	1.38^C	1.60^DE	1.11 ^DE	1.26^DE	4.76^B	5.90^B	7.52^A	2.21^CDE	2.03^CDE	1.88^CDE	0.23	0.001	0.001	0.001
PS	0.76^B	0.56^B	0.65^B	0.24^B	0.46^B	2.74^A	3.74^A	2.76^A	0.55^B	0.38^B	0.58^B	0.19	0.001	0.112	0.020
PI	1.08^A	0.77A	0.84^AB	0.83^AB	0.86^AB	0.79^ABC	0.78^ABC	ND	0.91^AB	0.24^C	0.79^ABC	0.11	0.001	0.001	0.001
PC	26.40^A	22.07^CD	24.17^BC	16.4^F	17.05^F	18.56^F	18.16^F	8.80^G	28.35^A	23.04^CDE	23.50^CD	0.44	0.001	0.002	0.001
Total	40.12^A	32.82^AB	31.59^AB	22.6^C	22.6^C	29.8^AB	31.30^AB	21.13^C	37.49^A	29.97^AB	30.69^AB	0.43	0.001	0.001	0.002

Abbreviations: PL + phospholipid; PA = phosphatidic acid; LDPG = lyso-diphosphatidylglycerol; CL = cardiolipin; LPE = lyso-phosphatidylethanolamine; LPS = lyso-phosphatidylserine; SM = sphingomyelin; PE = phosphatidylethanolamine; LPC = lyso-phosphatidylcholine; PS = phosphatidylserine; PI = phosphatidylinositol; PC = phosphatidylcholine; ND = not detected; and NS = not significant; Control = sample without incubation and protease pre-treatment; Inc = sample incubated at 45 °C without protease preparations; SEM = Standard error mean; E = effect of protease; EC = effect of protease; E*EC = interaction effect. Each value represents the mean of triplicate samples. Different superscript letters (A–E) in the same row indicate a significant difference (p < 0.05). The lipid was extracted from free dried hoki roe (20 g roe wet weight equivalent), which was hydrolysed with different amount of protease preparations (Alcalase: 200, 400, or 800 µL; HT: 200, 400, or 800 mg and FP-II: 200, 400, or 800 mg), corresponding to 1 %, 2 % and 4 %, respectively.