FIG. 3.

RT-PCR analysis for detection of fbpA, fbpAB, and fbpC transcripts in N. gonorrhoeae grown under iron-depleted and -replete conditions. Lanes: 1, DNA molecular size standards; 2, 4, 6, and 8, RNA obtained from N. gonorrhoeae under iron-depleted conditions; 3, 5, 7, and 9, RNA grown under iron-replete conditions; 2 and 3 RT-PCR with primer pairs SB3 and FBP(S) specific for fbpA; 4 and 5, RT-PCR with primer pairs FbpA.PR1 and FbpB.PR2 to amplify fbpA and the 5′ end of fbpB; 6 and 7, RT-PCR with primer pairs Fbp.C1 and Fbp.C2 to amplify fbpC; 8, RT-PCR with primer pairs RMP F1 and RMP R1 to amplify the rmp gene (positive control); 9, DNA control.