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Dehydration and carboxylesterase activity of kudzu 2-HID enzyme and mutants.
| carboxylesterase activity | dehydration activity | |
|---|---|---|
| (%) | (%) | |
| 2-HID | 100 | 100 |
| E89A | 23 ± 5.7 | 0 |
| E89Q | 50 ± 0.8 | 0 |
| T168A | 3 ± 0.5 | 1 ± 0.2 |
| S169A | 52 ± 1.1 | 0 |
| D269A | 0 | 3 ± 0.3 |
| H301A | 0 | 0 |
The relative activities to wild-type enzyme were obtained from identical incubations with equal amounts of protein, and the data are from 3 independent experiments.
