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. 2022 Nov 15;13:6957. doi: 10.1038/s41467-022-34698-4

Fig. 2. Detection TRM cells in herpes zoster-affected and contralateral human skin biopsies.

Fig. 2

Cryosections from rash site (HZ) and contralateral (Con) skin were analyzed for the expression of indicated markers by immunofluorescent staining. Total number and frequency of cells expressing the indicated markers were determined for each complete biopsy (A, B and E; data of individual subjects are shown in specific colors) and representative images are shown (C, D, F and G). Wilcoxon paired tests were used to compare HZ to con samples at the same time point: unadjusted 2-tailed p values are shown where p ≤ 0.05. C Donor 91, day 45 (D45). Scale bar = 50 µm. D Donor 78, D90, rash site. Scale bar = 20 µm. F Donor 84, D90, rash site. Scale bar = 20 µm. G Donor 91, D90, rash site. Scale bar = 20 µm. TIA-1, T cell-Restricted Intracellular Antigen-1; FOXP3, forkhead box P3; TRM, resident-memory T cells. Number of subjects tested per group: A D45 CON, D45 HZ, D90 CON, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 3. B D45 CON, n = 6; D45 HZ, D90 CON, n = 7, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 3. C D45 CON, n = 6; D45 HZ, D90 CON, n = 7, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 3. D D45 CON, n = 4; D45 HZ, n = 5; D90 CON and D90 HZ, n = 7; D360 CON, n = 3; D360 HZ, n = 2. E D45 CON, n = 6; D45 HZ, D90 CON, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 2. F D45 CON, D45 HZ, D90 CON, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 3. G. D45 CON, n = 5; D45 HZ, D90 CON, and D90 HZ, n = 7; D360 CON and D360 HZ, n = 3. Source data are provided as a Source Data file.