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. 2022 Nov 1;13:995791. doi: 10.3389/fimmu.2022.995791

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Copyright © 2022 Huang, Wang, Chen, Li, Zhu, Tang and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of MD2 on inflammatory injury and barrier function in IEC-6 enterocytes. (A) The expression of HMGB1, caspase-3, cleaved caspase-3, occludin and claudin-1 was measured by western blot analysis (n=3). (B) Cell monolayer permeability was detected using TEER. (C) Determination of FITC-D permeability (n=3). LPS+, IEC-6 + LPS; MD2-Over, MD2-overexpressing IEC-6 + LPS; MD2-IN-1+, IEC-6 + MD2 inhibitor + LPS; Vehicle, IEC-6 treated with DMSO at a 1:1000 dilution. p value was analyzed by one-way ANOVA with multiple comparisons, followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001.