Fig. 2. SRBSDV SP8 and RSV P2 provoke rapid degradation of SLR1.

a, b. Effects of viral proteins RBSDV SP8 and RSV P2 on the accumulation of SLR1 in N. benthamiana leaves. The co-infiltrated leaves were treated with MG132 (50 µM) or DMSO at 24 hpi and then were harvested for western blotting 24 h later. RbcL was used as a loading control. Each experiment was repeated three times with similar results. c, d. In vitro degradation assay. Total protein extracted from Nip seedlings was incubated in 10 µM GA₃ with approximately 50 µg purified TF-His, TF-His-SP8 (c) or TF-His-P2 (d) protein from E. coli at 30 °C incubator for indicated times, and these samples were collected at the indicated times for western blot using anti-SLR1 and anti-His. Anti-actin was used as a loading control. Each experiment was repeated three times with similar results. Phenotypes of Nip, SP8-ox (e) or P2-ox (g) seedlings treated with GA₃. Similar germinated seeds were planted in different concentrations of GA₃ (0, 0.1, 1, 2, 5, 10 µM) containing culture solution for about 7 d, n  =  3 biologically independent replicates per genotype. All images were photographed using a digital camera. Scale bar = 4 cm. Second leaf sheath lengths of Nip, SP8-ox (f) or RSV P2-ox (h) seedlings treated with GA_3. Values were obtained from n = 15 biologically independent plants. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. Source data including uncropped scans of gels (a–d) and p values of statistic tests (f and h) are provided in the Source data file.