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. 2022 Nov 14;13:6920. doi: 10.1038/s41467-022-34649-z

Fig. 5. SLR1 confers resistance to SRBSDV infection in rice.

Fig. 5

a Symptoms on LS, SLR1-GFP and RNAi-SLR1 plants 20 d after inoculation with SRBSDV. Values were obtained from n = 30 biologically independent plants, n = 3 biologically independent replicates per genotype. Scale bars=5 cm. b The percentages of SRBSDV symptomatic rice plants (%) in LS, SLR1-GFP and RNAi-SLR1. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. c Results of qRT-PCR showing the relative expression levels of SRBSDV RNAs (S2, S4 and S6) in infected LS, SLR1-GFP and RNAi-SLR1 rice plants. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. d The accumulation of SRBSDV CP protein in infected LS, SLR1-GFP and RNAi-SLR1 rice plants by western blot. RbcL serves as the loading control. e Symptoms on transgenic SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox plants 20 d after inoculation with SRBSDV. Values were obtained from n = 30 biologically independent plants, n = 3 biologically independent replicates per genotype. Scale bars=5 cm. f The percentages of SRBSDV symptomatic rice plants (%) in transgenic SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. g Results of qRT-PCR showing the relative expression levels of SRBSDV RNAs (S2, S4 and S6) in SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox rice plants. * at the top of columns indicate significant differences (p < 0.05) based on Fisher’s least significant difference tests. h Western blot showing the accumulation of SRBSDV CP protein in infected SLR1-GFP/Nip, SLR1-GFP/SP8-ox and SLR1-GFP/P2-ox rice plants. RbcL serves as the loading control. Source data including uncropped scans of gels (d and h) and p values of statistic tests (c and g) are provided in the Source data file.