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. 2022 Nov 11;58:102534. doi: 10.1016/j.redox.2022.102534

Fig. 3.

Fig. 3

The significance of SIRT1 in SRT1720-mediated protection against PQ-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. (A) Cells pretreated with 1 μM SRT1720 for 1 h were treated with or without 0.3 mM PQ over different intervals. Cells were then harvested and analyzed by Western blotting. The intensities in each group were subdivided by the internal control and normalized to the control group (100%). Data points represent the mean ± SD of three experiments. Differences between groups were evaluated through Kruskal-Wallis analysis of variance on ranks with the Student-Newman-Keuls test and considered significant at p < 0.05. *p < 0.05, compared to the control group. (B) After pretreatment with different dosages of sirtinol for 1 h, cells were treated with or without 0.3 mM PQ for another 24 h and then subjected to a neutral red survival assay. (C) After pre-inhibition of sirtinol for 30 min, cells were treated with SRT1720 for 1 h and then followed by 0.3 mM PQ treatment for another 24 h. Cells were subjected to a neutral red survival assay. (D) After SIRT1 silence, cells growing in 6-well plates were subjected to dot blot and Western blot analyses. The relative intensities of SIRT1 were subdivided by the internal control and normalized to the siControl group (100%). Data points represent the mean ± SD of at least three experiments. Differences between groups were evaluated through t-tests and considered significant at p < 0.05. (E) After SIRT1 silencing, cells growing in 96-well plates were subjected to a survival assay. Cell viability represents a percentage of neutral red assay results compared to controls. Data points represent the mean ± SD of at least three experiments. Differences between groups in raw data were evaluated through two-way ANOVA with the Student-Newman-Keuls tests and considered significant at p < 0.05. *p < 0.05, compared to the control group. #p < 0.05, compared to the PQ-treated group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)