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. 2022 Nov 14;13(11):956. doi: 10.1038/s41419-022-05419-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Microsomal fractions isolated from Huh7 and J7 cells were subjected to Vicia villosa (VVA)- or peanut agglutinin (PNA)-mediated pulldown (PD). Pulldown proteins were separated by 10% SDS-PAGE and stained by the colloidal blue silver stain. Protein bands with significant density changes were excised for LC/MS/MS identification. B, C Representative western blots demonstrating enriched PHB2 levels by VVA- or PNA-mediated pulldown in HCC cells with or without GALNT14 overexpression or silencing. D Western blotting following Tn antigen or MYC-tag immunoprecipitation (IP) using lysates of HCC cells transfected with the indicated plasmids. E Distribution of PHB2 and GALNT14 in microsomal fractions assessed by western blot using lysates of Huh7 and J7 cells with GALNT14 overexpression (upper) and silencing (lower). Calnexin was used as a specific marker for microsome fraction, while VDAC1 was used as a marker for the mitochondrial fraction. *Fold, fold of increase upon GALN14 (G14) overexpression.