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. 2022 Nov 14;12:19536. doi: 10.1038/s41598-022-23674-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Increased egg viability and mating frequency in the selected populations. (A,B) Egg viability measurement for each replicate population of FSB and FCB at 0–6 h (A) and 24–30 h (B) post heat shock and no shock treatment. n = 1 plate (200 eggs/plate) for each replicate population of FSB and FCB, and for heat shock and no shock treatments. (C) Post heat shock, the mating frequency was monitored every 30 min intervals for 36 h. n = 5 cages (100 males and females/cage) for selection regime and treatment group. Data are represented as mean ± SEM. ***p < 0.001.