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. 2022 Nov 14;13(11):955. doi: 10.1038/s41419-022-05409-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A, B Real‐time quantitative PCR analysis of the mRNA expression of fibrotic genes. C Immunofluorescence analysis of α‐SMA expression (scale bars = 275 μm). D Cell counting kit-8 (CCK-8) analysis of cell viability. E Western blot analysis of the protein expression of α‐SMA, Col1a1, and SIRT4. The data are reported as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 versus the respective control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, and ^####P < 0.0001 versus the NC + TGFβ1 group.