Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 14;12:19515. doi: 10.1038/s41598-022-23366-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Ultrasound Localization Microscopy and fUS Imaging Set Up. (a) Picture of the experimental setup. The animal is anesthetized and placed in a stereotaxic frame for the correct positioning of the US probe. After eye drop and mydriatic instillation, the eye is covered with ophthalmic coupling gel. The probe is tilted to image the plane shown in (d). (b) Scheme of the entire setup. The probe is connected to a functional ultrasound scanner. The microbubbles solution is a contrast agent administered intravenously via one of the rat tail lateral veins for ULM. The LED delivers light flashes for rfUS imaging and is triggered by the scanner through a microcontroller (Arduino) for precise timings. (c) Scheme of the rat eye, its principal structures, and the imaging field comprising the retinal vascular network. (d) Doppler image of a rat eye. The retinal vascular network is visible. (e) ULM scanning details. The scan starts at the initial position x_i in 200 µm steps along the mediolateral axis to the final position x_f. (f) Retinal fUS imaging stimulation design. The acquisition consists of a baseline recording of 45 s followed by 50 consecutive trials and terminated by a recovery period of 75 s, during which the RBV is continuously recorded. One trial consists of 15 s OFF, followed by a short light flash of 0.8 s ON and another 15 s OFF. One acquisition lasts 28 min. The mean trial is obtained via temporal averaging of the successive single trials. Figure created with BioRender.com.