Figure 7.

S1PR2 fate-mapped GC B cells and GC Tfh cells are recalled in boost immunization as GC B cells, GC-derived CD44⁺ memory B cells and plasma cells, or Tfh and Th1 cells, respectively

(A) S1pr2^ERT2−creRosa26^{stop-flox-RFP} mice recall experiment overview.

(B) Relative frequency of S1PR2-RFP⁺ fate-mapped mILN B cells at day 49.

(C) Relative frequency of S1PR2-RFP⁺ fate-mapped mILN GC B cells.

(D) Relative frequency of GC phenotype within S1PR2-RFP⁺ fate-mapped mILN B cells.

(E) Median flow cytometry plots of RBD⁺ S1PR2-RFP⁺ fate-mapped mILN B cell subsets.

(F) Relative frequency of RBD⁺ S1PR2-RFP⁺ fate-mapped mILN B cell subsets at days 14 and 49, respectively.

(G) Relative frequency of S1PR2-RFP⁺ fate-mapped plasma cells.

(H) Relative frequency of S1PR2-RFP⁺ fate-mapped CD4⁺ FOXP3⁻ cells.

(I) Day 49 median flow cytometry plots of S1PR2-RFP⁺ fate-mapped mILN FOXP3⁻ CD4⁺ cell subsets.

(J) Relative frequency of S1PR2-RFP⁺ fate-mapped mILN FOXP3⁻ CD4⁺ cell subsets.

(K) Relative frequency of S1PR2-RFP⁺ fate-mapped mILN Th1 (TBET⁺ CXCR3⁺ CXCR5⁻ PD1⁻ CD44⁺ CD62L⁻ FOXP3⁻ CD4⁺) cells.

For each time point, n = 5. For (C), (D), (H), and (K), the Mann-Whitney test was used. For (G), multiple Mann-Whitney tests per row were used, with p values corrected for multiple comparison analysis with the Holm-Šídák method. In (B)–(D), (G), (H), and (K), each symbol represents a biological replicate and the bar height the mean. In (F) and (J), bar heights represent mean, and error bars represent the standard deviation. Data are representative of two individual experiments.