REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
BUV661 Rat Anti-Mouse CD19	BD Biosciences	RRID: AB_2870243
APC/Fire™ 810 anti-mouse/human CD45R/B220 Antibody	BioLegend	RRID: AB_2860603
Alexa Fluor® 647 anti-mouse/human Bcl-6 Antibody	BioLegend	RRID: AB_2565299
Alexa Fluor® 700 anti-mouse Ki-67 Antibody	BioLegend	RRID: AB_2564285
BUV496 Rat Anti-Mouse IgD	BD Biosciences	Custom conjugation
IgM Monoclonal Antibody (II/41)	Thermo Fisher Scientific	RRID: AB_467582
Alexa Fluor® 594 anti-mouse CD38 Antibody	BioLegend	RRID: AB_2566435
Pacific Blue™ anti-IRF4 Antibody	BioLegend	RRID: AB_2814497
Brilliant Violet 510™ anti-mouse/human CD44 Antibody	BioLegend	RRID: AB_2650923
Brilliant Violet 785™ anti-mouse CD185 (CXCR5) Antibody	BioLegend	RRID: AB_2563981
PE/Dazzle™ 594 anti-T-bet Antibody	BioLegend	RRID: AB_2565677
PE/Cyanine5 anti-mouse CD69 Antibody	BioLegend	RRID: AB_313113
Brilliant Violet 711™ anti-mouse CD138 (Syndecan-1) Antibody	BioLegend	RRID: AB_2562571
BUV737 Rat Anti-Mouse CD62L	BD Biosciences	RRID: AB_2870155
APC anti-mouse CD184 (CXCR4) Antibody	BioLegend	RRID: AB_2562785
Brilliant Violet 605™ anti-mouse CD86 Antibody	BioLegend	RRID: AB_11204429
PerCP/Cyanine5.5 anti-mouse CD183 (CXCR3) Antibody	BioLegend	RRID: AB_1186017
BUV395 Hamster Anti-Mouse CD11c	BD Biosciences	RRID: AB_2738580
CD279 (PD-1) Monoclonal Antibody (RMP1-30), FITC	Thermo Fisher Scientific	RRID: AB_465467
FOXP3 Monoclonal Antibody (FJK-16s), PE-Cyanine5.5	Thermo Fisher Scientific	RRID: AB_11218094
PE/Fire(TM) 640 anti-mouse CD4 antibody	BioLegend	RRID: AB_2860585
PerCP/Cyanine5.5 anti-mouse CD38 antibody	BioLegend	RRID: AB_2563333
BUV615 Rat Anti-Mouse CD279 (PD-1)	BD Biosciences	RRID: AB_2875871
Alexa Fluor® 594 anti-T-bet Antibody	BioLegend	RRID: AB_2728474
Brilliant Violet 650™ anti-mouse CD183 (CXCR3) Antibody	BioLegend	RRID: AB_2563160
BUV615 Mouse Anti-Human CD19	BD Biosciences	RRID: AB_2875287
Spark NIR™ 685 anti-human CD20 Antibody	BioLegend	RRID: AB_2860775
APC/Fire™ 750 anti-human IgD Antibody	BioLegend	RRID: AB_2616988
Alexa Fluor® 488 anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody	BioLegend	RRID: AB_2563285
PE/Fire™ 640 anti-mouse CD38 Antibody	BioLegend	RRID: AB_2890673
PE/Fire™ 810 anti-mouse CD11c Recombinant Antibody	BioLegend	RRID: AB_2904307
PerCP anti-mouse/human CD44	BioLegend	RRID: AB_10639933
Alexa Fluor® 700 anti-mouse IgD Antibody	BioLegend	RRID: AB_2563341
Pacific Blue™ anti-mouse CD3 Antibody	BioLegend	RRID: AB_493645
Purified anti-mouse CD279 (PD-1) Antibody	BioLegend	RRID: AB_313418
Ki-67 Monoclonal Antibody (SolA15), FITC, eBioscience™	Thermo Fisher Scientific	RRID: AB_11151330
Goat anti-Rat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647	Thermo Fisher Scientific	RRID: AB_141778
CD21/CD35 Monoclonal Antibody (eBio8D9 (8D9)), Biotin, eBioscience™	Thermo Fisher Scientific	RRID: AB_466390
Goat Anti-Mouse IgG- Alkaline Phosphatase conjugate	Sigma-Aldrich	RRID: AB_11212223
Goat anti-mouse IgG1-Alkaline Phosphatase	Southern Biotech	RRID: AB_2794425
Goat anti-mouse IgG2a-Alkaline Phosphatase	Southern Biotech	RRID: AB_2794494
Goat Anti-mouse IgG2b-Alkaline Phosphatase	Southern Biotech	RRID: AB_2794541
Goat anti-mouse IgG2c-Alkaline Phosphatase	Southern Biotech	RRID: AB_2794461
Goat anti-mouse IgG3-Alkaline Phosphatase	Abcam	RRID: AB_10674160
Goat Anti-Human IgG Antibody, Alkaline Phosphatase conjugate	Sigma-Aldrich	RRID: AB_92427
Bacterial and virus strains
DH5α Competent Cells	Thermo Fisher Scientific	Cat#: 18265017
ChAdOx1 nCoV-19	this manuscript	Van Doremalan Nature volume 586, pages 578–582(2020)
ChAdOx1 OVA	this manuscript	N/A
Chemicals, peptides, and recombinant proteins
APC streptavidin	BioLegend	Cat#: 405207
Alexa Fluor® 647 Streptavidin	BioLegend	Cat#: 405237
Brilliant Violet 421™ Streptavidin	BioLegend	Cat#: 405225
PE Streptavidin	BioLegend	Cat#: 405204
PE/Cyanine7 Streptavidin	BioLegend	Cat#: 405206
Streptavidin, Alexa Fluor™ 750 conjugate	Thermo Fisher Scientific	Cat#: S21384
Biotin ≥99% (HPLC), lyophilized powder	Sigma-Aldrich	CAS Number: 58-85-5
Ni-NTA Agarose	Qiagen	Cat#: 30210
Tamoxifen	Sigma-Aldrich	CAS Number: 10540-29-1
Peanut oil	Sigma-Aldrich	CAS Number: 8002-03-7
Fixation Buffer	BD Biosciences	Cat#: 554655
Scigen O.C.T. Compound Cryostat Embedding Medium	Thermo Fisher Scientific	Cat#: 23-730-625
Normal Rat Serum	Sigma-Aldrich	Cat#: R9759
Goat serum	Sigma-Aldrich	Cat#: G9023-10ML
Bovine serum albumin	Sigma-Aldrich	CAS Number: 9048-46-8
TritonX-100	Sigma-Aldrich	CAS Number: 9036-19-5
Hydromount	National Diagnostics	Cat#: HS-106
Blocker Casein in PBS	Thermo Fisher Scientific	Cat#: 37528
Fetal Bovine Serum	Sigma-Aldrich	Cat#: F9665-500ML
Paraformaldehyde	Sigma-Aldrich	Cat#: P6148
Sucrose	Sigma-Aldrich	Cat#: S0389
p-Nitrophenyl Phosphate Substrate Buffer	Sigma-Aldrich	Cat#: 487664
SARS-CoV-2 FL-S protein	this manuscript	Graham et al.8
Critical commercial assays
EndoFree Plasmid Mega Kit	Qiagen	Cat#: 12362
Zenon™ Alexa Fluor™ 532 Mouse IgG1 Labeling Kit	Thermo Fisher Scientific	RRID: AB_2736944
PEI MAX® - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)	Polysciences	Cat#: 24765
eBioscience™ Foxp3/Transcription Factor Staining Buffer Set	Thermo Fisher Scientific	Cat#: 00-5523-00
Avidin/Biotin Blocking Kit	Vector laboratories	Cat#: SP-2001
HiLoad Superdex 200 pg 16/600 column	Sigma-Aldrich	GE28-9893-35
Amicon® Ultra-4 Centrifugal Filter Unit	Merck	Cat#: UFC801024
Deposited data
Experimental models: Cell lines
FreeStyle™ 293-F Cells	ThermoFisher Scientific	RRID: CVCL_D603
2.4G2 Hybridoma	ATCC	ATCC HB-197™
Experimental models: Organisms/strains
C57BL/6J-Rag2em3Lutzy/J	The Jackson Laboratory	RRID: IMSR_JAX:033526
Oligonucleotides
Recombinant DNA
SARS-CoV-2 Spike	BioBasic	Wuhan strain QHR63290.2
BirA plasmid	this manuscript	N/A
RBD-avi-His plasmid	this manuscript	N/A
pcDNA3.1	Invitrogen	Cat#: V79020
p8.91 plasmid (encoding for HIV-1 gag-pol)	this manuscript	N/A
CSFLW (lentivirus backbone expressing a firefly luciferase reporter gene)	this manuscript	N/A
Human Angiotensin-converting enzyme 2 (ACE2) expression plasmid	Addgene	Plasmid#1786; RRID: Addgene_1786
Software and algorithms
FlowJo v10.7	TreeStar	RRID: SCR_008520
R (version 4.0.2)	https://www.R-project.org/	RRID: SCR_001905
GraphPad Prism	GraphPad	RRID: SCR_002798
Glomax-Multi detection system	Promega	RRID: SCR_015575
Leica Application Suite X (LAS X)	Leica	RRID: SCR_013673
SOFTmax PRO	Molecular Devices	RRID: SCR_014240
ImageJ v1.53	https://imagej.nih.gov/ij/	RRID: SCR_003070