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. 2022 Nov 1;13:1054425. doi: 10.3389/fimmu.2022.1054425

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Copyright © 2022 Lamamy, Larue, Mariot, Dhommée, Demattei, Delneste and Gouilleux-Gruart

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD163, CD14, CD206 and CD16 analysis by flow cytometry during macrophage differentiation in GM-CSF or M-CSF. Human monocytes were cultured with 60 ng/ml M-CSF or GM-CSF for 6 days. (A) CD163, CD14, CD206 and CD16 expression was assessed by flow cytometry on day 0 in monocytes (MO) and day 6 in M-Mϕ and GM-Mϕ. Data are mean fluorescence intensity (MFI) from eight independent experiments (mean ± SD, N=8). (B) Results of flow cytometry of cytokine secretion in M-Mϕ and GM-Mϕ after lipopolysaccharide stimulation represented as a heatmap (mean ± SD, N=9). Mo, monocytes; M-Mϕ, M-CSF–induced macrophages; GM-Mϕ, GM-CSF–induced macrophages. ns = not significant, *p ≤ 0.05 and **p ≤ 0.01.