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Western blot analysis of protein levels of ATG5, ATG7 and LC3-I/II involved in autophagy and FcRn expression in macrophages. Human monocytes were cultured with 60 ng/ml M-CSF or GM-CSF for 6 days. (A) Proteins were extracted from M-Mϕ and GM-Mϕ on day 6. One representative blot is presented. (B) Quantification of FcRn, ATG5 and LC3-I/II expression normalized to GAPDH expression (mean ± SD, N=6). M-Mϕ, M-CSF–induced macrophages; GM-Mϕ, GM-CSF–induced macrophages. *p ≤ 0.05.
