Figure 2.

S0859 inhibits SLS-induced cytotoxicity in human keratinocytes, but not SLS-dependent hemolysis of erythrocytes. (A) Chemical structure of the N-cyanosulfonamide S0859. (B) Percent host cytotoxicity following 6 hour GAS infections of HaCaT cells (MOI 10) pre-treated for 1 hour with 100 µM S0859. Percent cytotoxicity was determined using an ethidium homodimer assay. DMSO was used as a vehicle control, and data are presented as mean ± sd, with n = 3 for each condition. (C) Growth curve of wild-type M1 5448 GAS in DMEM supplemented with 10% FBS in the presence or absence of 100 µM S0859. DMSO was used as a vehicle control, and un-inoculated DMEM + 10% FBS was used for each treatment condition as a sterility control. (D) Percent hemolysis (relative to 10% triton) induced by a 1 hour incubation of SLS preparations with erythrocytes in the presence of DMSO (Vehicle) or 50 µM S0859. *** = 0.0001 < P < 0.001, n.s. = not significant.